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- Simple in unicellular organisms, such as yeast and bacteria
- Only requires the inoculation of the appropriate medium
- Cloning rings used to clone distinct lineages of cell lines
- Single-cell suspension of cells exposed to a mutagenic agent is plated at high dilution to create isolated colonies
- Sterile cloning rings which have been dipped in grease are placed over an individual colony at an early growth stage
- Trypsin, an agent that breaks down proteins, is added
- Cloned cells are collected from the ring and transferred to a vessels for further growth
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