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The biological amplification of a specific DNA sequence through miotic division of a host cell
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| http://www.mun.ca/biology/scarr/Plasmid_cloning_summary.html |
- Used in a variety of biological experiments and practical applications, such as protein production
- Four steps:
- Fragmentation: DNA of interest is isolated to provide revelant DNA of the proper size
- Ligation: Vector is linearized by enzymes and incubated with fragment of interest with the enzyme DNA ligase
- Transfected: Vector with insert of interest is transfected into cells
- Screening/Selection: Transfected cells are cultured
- Term to know: amplification- an increase in the number of copies of a specific DNA fragment
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Definitions from The Centre for Cancer Education, University of Newcastle upon Tyne, Copyright
1997-2007
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